Table 3 Plasmids and strains
Plasmid | Description | References |
|---|---|---|
HO-poly-KanMX4-HO | Vector for targeted integration at the HO locus | [36] |
pRS416 | pBluescript II SK + , URA3, CEN6, ARSH4 | [42] |
pRH274 | pRS416 + PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 | [43] |
pRH277 | HO-poly-KanMX4-HO + PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 | [35] |
pRH1015 | PRS416 + [pho13 homology]—PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7—[pho13 homology] | This study |
Strains | Genotype | References |
|---|---|---|
BY4741 | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 | [18] |
CEN.PK2-1C | MATa ura3-52 trp1-289 leu2-3_112 his3Δ1 MAL2-8C SUC2 | EUROSCARF |
YB-2625 | S. cerevisiae Diploid isolated from bagasse | ARSa |
YRH1943 | MAT a Haploid 1B isolated from YB-2625 Tetrad #1 | This study |
YRH1944 | MAT alpha Haploid 2A isolated from YB-2625 Tetrad #2 | This study |
YRH1945 | MAT alpha Haploid 3B isolated from YB-2625 Tetrad #3 | This study |
YRH1946 | MAT alpha Haploid 4B isolated from YB-2625 Tetrad #4 | This study |
YRH2066 | YRH1946 pho13∆::PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 | This study |
YRH2073 | BY4741 pho13∆::PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 | This study |
YRH2074 | CEN.PK2-1C pho13∆::PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 | This study |
YRH2121 | YRH1946 ho∆::PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7; KanMX | This study |