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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Development of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production

Fig. 2

The engineered base-editing system enables C-to-T conversion in A. succinogenes. a Architectures of CBEs. rAPOBEC1-nCas9(D10A), rAPOBEC1-dCpf1(D917A), and rAPOBEC1-dCpf1(D917A-E1006A), a fusion protein composed of a deaminase rAPOBEC1 at the N terminus, and nCas9(D10A)/dCpf1(D917A)/dCpf1(D917A-E1006A) at the C terminus; rAPOBEC1-nCas9(D10A)-UGI/rAPOBEC1-dCpf1(D917A)-UGI/rAPOBEC1-dCpf1(D917A-E1006A)-UGI, a fusion protein composed of a deaminase rAPOBEC1 at the N terminus, nCas9(D10A)/dCpf1(D917A)/dCpf1(D917A-E1006A) at the middle, and UGI at the C terminus. b The rAPOBEC1-nCas9(D10A)-UGI enables C-to-T base-editing targeting lacZ in A. succinogenes. c Architectures of optimized CBEs. d The subculture improves gene editing efficiency (the strains were streaked on TSB-X-gal plate). The red dots were the marked white colonies

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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