Fig. 3

The window and efficiency of the optimized base-editing system enables C-to-T / A-to-G conversion. a The editing region of the targeting gene by the optimized base-editing system. The red letters were the positions of successful editing. b Editing efficiency of the optimized base-editing system enables C-to-T conversion by different sgRNAs in A. succinogenes. The graph shows the percentage of total DNA sequencing reads containing T at each of the successful editing target C positions by the optimized CBE. c Architectures of the ABEs. d The editing region of the targeting gene by the ABE. The red letters were the positions of successful editing. e Editing efficiency of the base-editing system enables A-to-G conversion by different sgRNAs in A. succinogenes. The graph shows the percentage of total DNA sequencing reads containing G at each of the successful editing target A positions by ABE