Fig. 1
From: Four ways of implementing robustness quantification in strain characterisation
Overview of oxygen-limited screening and strain performance in various lignocellulosic hydrolysates. a Yeast can metabolise sugars through fermentation or respiration depending on oxygen and substrate availability. Using a glycerol trap loop to close the cultivation flask blocked the diffusion of oxygen from outside, causing the cells to rely exclusively on the oxygen trapped inside. Hence, yeast employed a respiro-fermentative metabolism until oxygen was available, but switched to a fermentative metabolism thereafter. b Overview of five functions (specific growth rate, lag phase, ethanol/glycerol/cell mass yields) of three S. cerevisiae strains (CEN.PK113-7D, Ethanol Red, PE2) in seven different lignocellulosic hydrolysates at 50% (vol/vol) and a control condition (Delft). Shades of green refer to non-woody hydrolysates, while shades of purple refer to woody hydrolysates