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Table 3 Kinetic parameters of SaRohS and important mutants compared with KaRohS

From: Characterization of an efficient N-oxygenase from Saccharothrix sp. and its application in the synthesis of azomycin

 

KaRohS

SaRohS

T75A

G95A/K115T

kcat (min−1)

0.23 ± 0.005

0.42 ± 0.007

0.051 ± 0.003

1.11 ± 0.005

Km (µM)

390.55 ± 0.02

182.69 ± 0.01

924.17 ± 0.10

82.68 ± 0.001

kcat/Km (µM−1 min−1)

0.6 × 10–3

2.3 × 10–3

0.06 × 10–3

13.4 × 10–3

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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