Fig. 2

Clr-2 is the transcriptional activator of lignocellulase in P. funiculosum. a Schematic representation of expression cassettes of ctf1a, clr-2, and ctf1b. b Transcript levels of ctf1a, clr-2, and ctf1b reflected in their respective overexpressed strains by semiquantitative PCR. The lower panel shows the technical replicates of tubulin expression in all the strains. c Transcript levels of ctf1a (left), ctf1b (middle), and clr-2 (right) in their respective overexpressed strains measured by real-time PCR in the corresponding transformants. d Total cellulase activity of secretomes measured by filter paper unit (FPU) assay. e Transcript levels of cellulases in P_clr2Clr-2/NCIM1228 relative to NCIM1228 when grown in MM glucose and MM cellulose for 72 h