Fig. 3
Effect of clr-2 overexpression in ∆Mig1. pPclr2Clr-2 was transformed into ∆Mig1, and the secretome of the resultant strain, Pclr2Clr-2/∆Mig1, was evaluated for a exocellulase (Avicelase), b endocellulase (CMCase), c β-glucosidase (pNPGase), and d xylanase along with the secretomes of NCIM1228, Pclr-2Clr-2/NCIM1228, and ∆Mig1. e Total cellulase activity of the Pclr2Clr-2/∆Mig1 secretome compared with parent strains by FPU assay. f Total protein present in the secretomes was measured by the BCA method. The biomass hydrolyzing capacity of secretomes was determined by incubating 20% nitric acid-pretreated rice straw biomass (by dry biomass weight (DBW)) with secretomes at a 30 mg/ml concentration for 72 h. The supernatant collected was used to determine glucose g and xylose h release by HPLC. Percentage hydrolysis i was calculated by dividing the total sugar released during hydrolysis by the total sugar content present in the dry biomass