Fig. 5

Posttranscriptional events leading to cellulase production are Ca²⁺ dependent. a Dry mycelial weight of 24-h cultures of NCIM1228 and ∆Mig1 in Mandel's medium containing 4% glucose and 4% cellulose with and without 50 mg/l CaCl₂. b Impact of calcium on transcript levels of important cellulases in NCIM1228 and ∆Mig1 grown in glucose (30 h) and cellulose (60 h) by RT‒PCR. c SDS‒PAGE, in-gel MUG β-glucosidase zymogram, and Western blot with anti-Cbh1 of NCIM1228 and ∆Mig1 secretome showing the effect of calcium on total secretome profile, β-glucosidase and Cbh1 levels, respectively. NCIM1228 and ∆Mig1 secretomes were also examined for d total cellulase activity by filter paper unit (FPU) assay, e exocellulase (cellulase), f endocellulase (CMCase), g β-glucosidase (pNPGase), h xylanases, and i total amylase activity. All experiments were repeated three times; statistical significance was determined by a one-tailed, unequal variance t test