Fig. 6

Ssp1 is a carbon-derepressing Ca²⁺-activated kinase. a Heatmap of differentially regulated Ca²⁺-activated kinases identified in NCIM1228 and ∆Mig1. b An increase in transcript levels of ssp1 in NCIM1228 and ∆Mig1 was determined by RT‒PCR in cellulose relative to glucose. c Schematic of the ssp1 expression cassette. d Confirmation PCR of ssp1 expression cassette integration. Transcript levels of ssp1 when Ssp1 was overexpressed in e NCIM1228 and f ∆Mig1. g SDS‒PAGE profile and h total cellulase activity present in the secretomes of parent and engineered strains grown in cellulosic RCM medium. i Transcript levels of major cellulases in Ssp1/∆Mig1 compared to ∆Mig1 in MM medium containing cellulose. j Transcript levels of ssp1 in cellulose + Ca.²⁺ relative to cellulose. k Total cellulase activity of Ssp1/∆Mig1 and P_cbh1Clr-2/Ssp1/∆Mig1 determined by FPU assay when the strains were grown in RCM medium with 5 g/l CaCO₃