Fig. 7
Enhanced induction of cellulase at transcriptional and translation and secretory levels. We achieved a > sevenfold increase in cellulase yield compared with the parent strain NCIM1228 by (i) strategic removal of catabolite repression (∆Mig1), ii clr-2 overexpression through a positive feedback loop, iii increased Ca.2+ concentration in the induction medium, and iv ssp1 overexpression. These changes weakened catabolite repression and enhanced transcriptional induction and the translation and secretion of cellulase in P. funiculosum NCIM1228