Fig. 1

A Anaerobic growth of Z. mobilis ZM4 in minimal media containing varying amounts of added ethanol (0.3, 0.6, or 0.8 M) or isobutanol (0.1 and 0.15 M). Z. mobilis was more susceptible to growth inhibition by isobutanol than ethanol. Each data point represents the average of four biological replicates, with error bars indicating the standard deviation. Refer to Table S2 for doubling times and methods section for cell density measurements. B Bright-field microscopy evaluation of gross morphological alterations when Z mobilis was grown in minimal media supplemented with either ethanol (0.3, 0.6, or 0.8 M) or isobutanol (0.10 or 0.15 M)