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Table 1 RCCD experimental design developed using Design-Expert version 7.0.0 software

From: Bioprospecting CAZymes repertoire of Aspergillus fumigatus for eco-friendly value-added transformations of agro-forest biomass

Run no.

Factor A pH

Factor B temperature

Factor C time

Different responses

CMCase (IU/gds)

Xylanase (IU/gds)

β-Glucosidase (IU/gds)

FPase (IU/gds)

Observed

Predicted

Observed

Predicted

Observed

Predicted

Observed

Predicted

1

7

20

5

42.92

42.50

681.76

688.47

47.48

22.99

23.74

22.99

2

11

20

5

50.08

50.11

651.35

658.15

41.70

20.22

20.85

20.22

3

7

40

5

126.31

126.34

1434.66

1429.54

69.35

34.47

34.68

34.47

4

11

40

5

121.90

121.75

548.64

555.37

74.34

36.06

37.19

36.06

5

7

20

9

80.40

80.43

853.34

847.35

54.87

27.45

27.43

27.45

6

11

20

9

82.26

82.11

749.18

755.04

53.95

26.35

27.25

26.35

7

7

40

9

118.76

118.60

1315.1

1309.05

61.37

30.06

30.54

30.06

8

11

40

9

107.77

108.06

378.86

372.89

67.97

33.30

33.66

33.30

9

5.64

30

7

91.15

91.40

1361.96

1368.54

61.54

30.95

30.64

30.95

10

12.36

30

7

89.01

88.94

563.46

555.83

60.28

31.35

30.09

31.35

11

9

13.18

7

44.56

44.81

419.06

411.47

31.57

16.61

15.79

16.61

12

9

46.82

7

137.21

137.14

706.74

713.28

62.93

32.11

31.34

32.11

13

9

30

3.64

81.91

82.14

976.24

967.61

57.77

29.97

28.89

29.97

14

9

30

10.36

102.60

102.54

940.19

947.77

61.83

31.41

30.92

31.41

15

9

30

7

89.90

89.95

833.21

834.59

54.77

26.62

27.39

26.62

16

9

30

7

89.90

89.95

846.26

834.59

55.00

26.62

27.50

26.62

17

9

30

7

90.34

89.95

831.34

834.59

53.23

26.62

26.61

26.62

18

9

30

7

90.36

89.95

832.59

834.59

52.77

26.62

26.38

26.62

19

9

30

7

89.77

89.95

833.21

834.59

52.14

26.62

26.07

26.62

20

9

30

7

89.44

89.95

830.72

834.59

52.96

26.62

26.01

26.62