Pretreatment method | Source of enzyme used for saccharification | Temperature (°C) | Enzyme loading (per g of wheat straw) | Reducing sugars (mg/g wheat straw) | Reference |
---|---|---|---|---|---|
Acetic acid/steam explosion/supercritical carbon dioxide pretreatment | Commercial cellulase (ASA Spezialenzyme GmbH, Germany) | 50 | 15 FPU | 336.41 | [30] |
Hydrothermal pretreatment | Cellic CTec2 (Sigma-Aldrich, Germany) | 50 | 100 CMCase | 480.00 | [31] |
Ammonium sulfite pretreatment with sodium carbonate supplementation | Commercial cellulase and xylanase (Imperial Jade Bio-Technology Co., Ltd. Yinchuan, China) | 50 | 35 FPU cellulase and 70 FXU xylanase | 413.00 | [53] |
[EMIM][Ac] Ionic liquid pretreatment | Sporotrichum thermophile xylanase | 40 | 160 U | 281.00 | [33] |
Acid pretreatment | T. reesei NCIM 1186 | 50 | 30.85 CMCase | 371.44 | [54] |
Alkali pretreatment | Phoma exigua secretome | 55 | 15.23 FPU | 101.16 | [34] |
Acid pretreatment | Penicillium janthinellum EMS-UV-8 cellulase | 50 | 20 FPU | 376.00 | [55] |
Alkali pretreatment | Aspergillus fumigatus | 50 | 30 FPU | 675.36 | Present study |