Fig. 3

Cel7A enzymes bind to immobilized cellulose but not to lignin. First column: interference reflection microscopy images of synthetic lignocellulose immobilized on a glass coverslip. Second column: total internal reflection fluorescence microscopy images of Qdot-labeled Cel7A as they bind to cellulose over 500 s. Third column: lignin fluorescently stained by the lignin dye Basic Fuchsin. Fourth column: overlay of Qdot-labeled Cel7A (green) and lignin (magenta) images. Fifth column: cropped regions of the overlay images shown in the fourth column. For columns 1–4: scale bar = 10 µm. For column 5: scale bar = 1 µm. Sixth column: Pearson's correlation coefficient value between Cel7A and lignin