Fig. 1
From: Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Directed evolution for thermosensitive repressor cI857. a Working principle of λPR-cI857 system. In the expression cassette, the repressor gene cI857 was constitutively expressed. The inducible promoter PR controlled the expression of EGFP. b General schematic procedure for the directed evolution of cI857. c Fluorescence intensity of the sorted 768 cells after fermentation for 6 h in 96 deep well plate. d Fluorescence intensity of mutant M1 and wild-type strain after 9 h of fermentation in the shake flask at 30 ℃/37 ℃