Fig. 5
From: Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Resveratrol-responsive biosensor in E. coli. a Schematic diagram of the construction of the resveratrol biosensor and high-throughput screening method. TtgR protein specifically responds to resveratrol produced by exogenous biosynthetic pathway, thus controlling the expression of mCherry. STS, stilbene synthase; 4CL, p-Coumarate:CoA ligase. mCherry fluorescence of strain BL21 (DE3) harboring the pTtgR construct induced by varying concentrations of p-Coumaric acid (b) and resveratrol (c). d Resveratrol production of strain BL21 (DE3) harboring pETDuet–Pgap–STS–4CL plasmid and pTtgR construct. e Characterization of the biosensor response to intracellular resveratrol biosynthesis in strain MM. Strains were cultured with exogenous addition of 900 mg/L p-coumaric acid at 30 °C