Fig. 6
From: Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Ultrahigh-throughput screening of improved resveratrol producing strains. a Fluorescence intensity of strains sorted by flow cytometry. Strains were cultured in 24 deep well plates containing 900 mg/L of p-coumaric acid. Black line represents the fluorescence intensity of the starting strain. b Resveratrol productions of retransformed strains cultured in shake flasks at 30 °C. c RT-qPCR analysis of the control strain or cells carrying the mpETDuet plasmid expressing STS. d Fluorescence intensity of strain BL21 (DE3) harboring pETDuet or mpETDuet plasmid expressing EGFP. RT-qPCR analysis (e) and resveratrol production assay (f) of strains harboring the test plasmids. *P < 0.05, **P < 0.01, ***P < 0.001; two-sided student’s t test