Table 1 Mutation spectrum at TAA of wild-type strain with Pol I* plasmid (β-lactamase as reporter)
From: Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Nucleotide changes | Amino acid changes | Count | Frequency (%) |
|---|---|---|---|
TAA → TTA | STOP → L | 30 | 61.2 |
TAA → TCA | STOP → S | 9 | 18.4 |
TAA → CAA | STOP → Q | 7 | 14.3 |
TAA → CTA | STOP → L | 1 | 2 |
TAA → TAA | STOP → STOP | 2 | 4 |
Total | – | 49 | 100 |