Table 2 Mutation spectrum at TAA of MutS60 with Pol I* plasmid (aadA as reporter)
From: Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Nucleotide changes | Amino acid changes | Count | Frequency (%) |
|---|---|---|---|
TAA → CAA | STOP → Q | 41 | 85 |
TAA → AAA | STOP → K | 5 | 11 |
TAA → TAC | STOP → Y | 2 | 4 |
Total | – | 48 | 100 |