Skip to main content

Table 1 Strains and plasmids used in this study

From: The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate

Name

Relevant genotype

References

Cyanobacterial strains

 Synechocystis sp. PCC 6803

Wild type

Pasteur culture collection

 Δadc1

Δadc1 knockout, KmR inserted between adc1 gene in Synechocystis genome

[5]

 WT control (WTc)

WT, CmR integrated at flanking region of psbA2 gene in Synechocystis genome

This study

 Δadc1 control (Δadc1c)

Δadc1, CmR integrated at flanking region of psbA2 gene in Synechocystis genome

This study

 OXP

ProC, CmR integrated at flanking region of psbA2 gene in Synechocystis WT genome

This study

 OXP/Δadc1

ProC, CmR integrated at flanking region of psbA2 gene in Synechocystis Δadc1 genome

This study

Plasmids

 pEERM

PpsbA2- CmR; plasmid containing flanking region of psbA2 gene

[26]

 pEERM_ProC

PpsbA2-ProC- CmR; integrated between SpeI and PstI sites of pEERM

This study

  1. PpsbA2, psbA2 promoter; CmR, chloramphenicol resistance cassette