Table 3 Strains and plasmids used in this study
Strain or plasmid name | Description | Source or reference |
|---|---|---|
Strains | ||
 Escherichia coli | ||
  HST08 | Cloning host | TaKaRa |
  Top10 | Modification host | Invitrogen |
Moorella thermoacetica | ||
 ATCC39073 | Wild-type strain | ATCC |
 ∆pyrF | pyrF gene was deleted | 33 |
 pduL2::sadh | sadh gene with G3PD promoter was introduced into the pduL2 region | This study |
 pduL2::IPA | The thermophilic IPA operon was introduced into the pduL2 region | This study |
Plasmids | ||
 pBAD33 | Backbone plasmid for methylation plasmids | 34 |
 pBAD-M1281 | pBAD33 carrying the Moth_1671, Moth_1672, and Moth_2281 for DNA methylation | 33 |
 pK18mob | Backbone plasmid for transformation plasmids | 35 |
 pK18-∆pduL2::ldh | A transformation plasmid to introduce ldh with G3PD promoter into the pduL2 region | 36 |
 pHM19 | sadh was cloned in pK18-∆pduL2::ldh, replacing ldh | This study |
 pK18-kan2 | Backbone plasmid for pHM35 construct | 37 |
 pHM35 | The promoter region of cao was cloned | This study |
 pHM36 | The promoter region of cao and sadh were cloned | This study |
 pHM5 | A transformation plasmid to introduce the thermophilic acetone operon into the pduL2 region with pyrF marker | 12 |
 pHM71 | A transformation plasmid to introduce the thermophilic IPA operon into the pduL2 region with pyrF marker | This study |