Fig. 1

Α comparative analysis of total product release (in nC * min) resulting from cellulose oxidation by TthLPMO9G (grey), following a 16-h reaction period. The same reaction was also conducted in the presence of catalase (red). Each reaction incorporated a 0.1% w/v PASC concentration, 4 μΜ TthLPMO9G, and 1 mM electron donor, and analyzed via HPAEC-PAD. All experiments were carried out in 50 mM sodium acetate buffer, pH 6.0, at 45 °C. The chromatograms on the left delineate all eluted products for the 13–19 min retention window. Bars denote mean values, with error bars indicating the standard error derived from two independent experiments, each performed at least twice