Fig. 4

Representation of the AlphaFold model for TthLPMO9G rational design mining of selecting Ser28 as a mutation substitution location. The active site is prominently showcased, with focus on the location where Ser28 is situated relative to the adjacent histidine His1. Active site amino acids are colored in red, while residues within a 5 Å radius around His1 are in yellow. The dashed blue line represents the distance measured using the AlphaFold wizard. Distances are derived from the nearest atoms between these specific residues