Fig. 5

AlphaFold model representation of TthLPMO9G, specifically showcasing the active site and portions of the second coordination sphere where Ser28 and His140 are located. The active site and relevant surrounding residues were visualized and analyzed using the PyMOL molecular visualization system. The lower image shows the modeled single-point mutations S28A and H140A. Single-point mutations were introduced in silico using the mutagenesis wizard in PyMOL, which allows for the substitution of selected amino acids. Distances between the nitrogen atoms of the mutated residues and the copper ion in the active site were measured within PyMOL to assess changes in the coordination sphere resulting from the mutations