Fig. 6

Comparative analysis of total product release (in nC*min) resulting from cellulose oxidation by TthLPMO9G variants, following 4 h (dark grey) and 16 h (light striped bars) reaction periods using various electron donors. The experiment comprises the WT TthLPMO9G and two mutations, S28A and H140A. Each reaction incorporated a 0.1% w/v PASC concentration, 4 μΜ LPMO and 1 mM electron donors. All experiments were carried out in 50 mM sodium acetate buffer, pH 6.0, at 45 °C. The bar chart illustrates the comparative data of eluted products for WT and the mutants with each respective electron donor. The measured areas correspond to the peaks between 13–19 min, which is the elution window for C1 oxidized products. The bars represent mean values, with error bars indicating the standard error derived from two independent experiments, each conducted at least twice. All control reactions without enzyme addition resulted in zero area