Table 2 Oxidase and peroxidase activity assays for TthLPMO9G and its point mutations
Assay | Activity | Notes | ||
|---|---|---|---|---|
WT | S28A | H140A | ||
Amplex® Reda | 1.67 ± 0.2 (U/mg) | 1.37 ± 0.1 (U/mg) | 1.01 ± 0.1 (U/mg) | A decrease of 18% and 40% was observed in activity, respectively, compared to the WT |
2,6-DMPb | 11.5 ± 0.5 (U/g) | 10.6 ± 0.4 (U/g) | 8.3 ± 0.2 (U/g) | A decrease of 8% and 28% was observed in activity, respectively, compared to the WT |