Fig. 4From: Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiberAnalysis of extracellular proteins and CMCase, pNPGase, xylanase and amylase zymogram of different engineered strains after 120 h of cultivation. A SDS-PAGE of extracellular proteins of M12 and its engineered strains without pyrG marker. B SDS-PAGE of extracellular proteins of four engineered strains with pyrG marker and corresponding pyrG-free strains. Enzymatic analysis of fermentation broth of five strains. C–F Active zymogram analysis of CMCase, pNPGase, xylanase and amylase of M12 and its engineered strainsBack to article page