Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile

Table 3 Main fatty acid composition in TAG and PLs of wild-type strains and △PLD1 strains

Culture condition	Lipid class	C14:0	Percent of fatty acids (%)						PUFAs
Culture condition	Lipid class	C14:0	C16:0	C18:0	SFAs	EPA	DPA	DHA	PUFAs
WT 72 h	TAG	0.12 ± 0.04	52.0 ± 3.53	2.43 ± 0.71	54.6 ± 4.26	0.14 ± 0.03	5.64 ± 0.82	28.8 ± 0.5	34.6 ± 0.59
WT 72 h	PL	2.03 ± 0.9	34.8 ± 13.57	13.7 ± 2.55	50.5 ± 15.1	0.00 ± 0.00	3.18 ± 0.44	11.1 ± 2.07	14.3 ± 1.63
△PLD1 72 h	TAG	0.17 ± 0.03	44.0 ± 4.75	2.57 ± 0.41	46.7 ± 4.72	0.18 ± 0.03	7.71 ± 0.63	32.6 ± 4.88	40.5 ± 5.52
△PLD1 72 h	PL	2.55 ± 0.35	28.9 ± 1.04	17.0 ± 0.83	48.5 ± 1.38	0.00 ± 0.00	1.05 ± 0.44	3.81 ± 0.23	4.86 ± 0.67
WT 120 h	TAG	0.30 ± 0.09	46.9 ± 2.04	2.86 ± 0.32	50.1 ± 2.45	0.22 ± 0.06	6.02 ± 0.80	30.7 ± 2.27	36.9 ± 1.53
WT 120 h	PL	1.26 ± 0.16	25.7 ± 12.91	13.2 ± 0.49	40.2 ± 12.6	0.00 ± 0.00	3.09 ± 1.23	6.69 ± 0.72	9.78 ± 0.94
△PLD1 120 h	TAG	0.26 ± 0.21	42.6 ± 1.01	1.67 ± 0.51	44.5 ± 1.17	0.20 ± 0.08	7.75 ± 1.04	34.0 ± 1.70	42.0 ± 0.62
△PLD1 120 h	PL	2.30 ± 0.83	20.9 ± 2.99	11.2 ± 2.15	34.4 ± 5.77	0.00 ± 0.00	0.57 ± 0.21	3.22 ± 0.75	3.79 ± 0.72

ISSN: 2731-3654