Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile

Zhang, Yiting; Cui, Xiaowen; Lin, Shuizhi; Lu, Tao; Li, Hao; Lu, Yinghua; Cao, Mingfeng; Lin, Xihuang; Ling, Xueping

doi:10.1186/s13068-024-02465-w

Biotechnology for Biofuels and Bioproducts

Table 4 Phospholipid species in S. limacinum SR21 (120 h)

From: Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile

Type	Unsaturation	Acyl composition	Molecular formula	m/z	Content (μg/g)
PC	C30:0	C14:0/C16:0	C₃₈H₇₆O₈NP	750.5285	6.45
	C32:0	C16:0/C16:0	C₄₀H₈₀O₈NP	778.5598	16.10
	C36:5	C14:0/C22:5	C₄₄H₇₈O₈NP	824.5442	15.53
	C36:6	C14:0/C22:6	C₄₄H₇₆O₈NP	822.5285	18.19
	C37:5	C15:0/C22:5	C₄₅H₈₀O₈NP	838.5598	6.45
	C37:6	C15:0/C22:6	C₄₅H₇₈O₈NP	836.5442	19.19
	C38:5	C16:0/C22:5	C₄₆H₈₂O₈NP	852.5755	661.44
	C38:6	C16:0/C22:6	C₄₆H₈₀O₈NP	850.5598	2076.51
	C40:6	C18:0/C22:6	C₄₈H₈₄O₈NP	878.5911	14.32
	C40:7	C18:1/C22:6	C₄₈H₈₂O₈NP	876.5755	28.22
	C42:10	C20:4/C22:6	C₅₀H₈₀O₈NP	898.5598	124.96
	C42:11	C20:5/C22:6	C₅₀H₇₈O₈NP	896.5442	270.73
	C44:10	C22:5/C22:5	C₅₂H₈₄O₈NP	926.5911	58.27
	C44:11	C22:5/C22:6	C₅₂H₈₂O₈NP	924.5755	525.61
	C44:12	C22:6/C22:6	C₅₂H₈₀O₈NP	922.5598	1518.21
LPC	C14:0	C14:0	C₂₂H₄₆NO₇P	512.2988	4.19
	C15:0	C15:0	C₂₃H₄₈NO₇P	526.3145	1.30
	C16:0	C16:0	C₂₄H₅₀NO₇P	540.3301	59.88
	C18:0	C18:0	C₂₆H₅₄NO₇P	568.3614	1.10
	C18:1	C18:1	C₂₆H₅₂NO₇P	566.3458	2.79
	C18:2	C18:2	C₂₆H₅₀NO₇P	564.3301	2.28
	C20:5	C20:5	C₂₈H₄₈NO₇P	586.3145	2.44
	C22:5	C22:5	C₃₀H₅₂NO₇P	614.3458	109.23
	C22:6	C22:6	C₃₀H₅₀NO₇P	612.3301	489.56
PG	C30:0	C14:0/C16:0	C₃₆H₇₁O₁₀P	693.4707	0.35
	C31:0	C15:0/C16:0	C₃₇H₇₃O₁₀P	707.4863	1.20
	C32:0	C16:0/C16:0	C₃₈H₇₅O₁₀P	721.502	147.72
	C38:5	C16:0/C22:5	C₄₄H₇₇O₁₀P	795.5176	11.97
	C38:6	C16:0/C22:6	C₄₄H₇₅O₁₀P	793.502	98.12
	C40:6	C18:0/C22:6	C₄₆H₇₉O₁₀P	821.5333	0.80
	C40:7	C18:1/C22:6	C₄₆H₇₇O₁₀P	819.5176	1.34
	C42:10	C20:4/C22:6	C₄₈H₇₅O₁₀P	841.502	3.60
	C44:11	C22:5/C22:6	C₅₀H₇₇O₁₀P	867.5176	2.57
	C44:12	C22:6/C22:6	C₅₀H₇₅O₁₀P	865.502	5.03
PI	C38:5	C16:0/C22:5	C₄₇H₈₁O₁₃P	883.5337	1069.19
	C38:6	C16:0/C22:6	C₄₇H₇₉O₁₃P	881.518	2005.56
PE	C38:5	C16:0/C22:5	C₄₃H₇₆O₈NP	764.523	74.82
	C38:6	C16:0/C22:6	C₄₃H₇₄O₈NP	762.5074	211.59
	C44:11	C22:5/C22:6	C₄₉H₇₆O₈NP	836.523	20.26
	C44:12	C22:6/C22:6	C₄₉H₇₄O₈NP	834.5074	56.60
PS	C28:0	C14:0/C14:0	C₃₄H₆₆NO₁₀P	678.4346	63.49
	C44:12	C22:6/C22:6	C₅₀H₇₄NO₁₀P	878.4972	12.93
PA	C44:12	C22:6/C22:6	C₄₇H₆₉O₈P	791.4652	180.21

Back to article page

ISSN: 2731-3654

Contact us

General enquiries: journalsubmissions@springernature.com