Fig. 3

Xylose reductase from Scheffersomyces xylosifermentans lacks a cofactor preference. A XR and XDH activities under moderate aeration and high aeration conditions expressed in units (U) per mg protein [U (mg protein)⁻¹]. B Production of ethanol, xylitol, and/or glycerol by the species tested for XR and XDH activities (reproduced from Fig. 1). Colored squares: metabolite(s) mainly produced by each species based on yields. Gray squares: the species produced low yields or did not produce the metabolite. Spa—Spathaspora passalidarum, Sxy—Scheffersomyces xylosifermentans, Scoip—Scheffersomyces coipomoensis, Sama—Scheffersomyces amazonensis, and Scer—Saccharomyces cerevisiae (negative control). Error bars indicate the standard deviation from the three biological replicates. Asterisks denote significant differences between the activities on NADH and NADPH for each species (P < 0.05)