Fig. 4

Cellulase production in the xyr1-A824V overexpressing strains QA1Xg and QA1Xc under Avicel culture conditions. A Qualitative evaluation of the ability to secrete cellulase using the MM plate containing 0.5% ball-milled Avicel as sole carbon source. B SDS-PAGE analysis of the fermentation supernatants from QA1Xg, QA1Xc and the parental strain QM53 under Avicel culture conditions. C The activities of FPA and D the amount of extracellular protein at 3 days, 5 days, and 7 days under Avicel culture conditions, respectively. E The activities of CBH, EG and BGL at 7 days under Avicel culture conditions. F The transcript levels of the cellobiohydrolase genes cbh1 and cbh2. G The transcript levels of the endoglucanase genes eg1 and eg2. H The transcript level of the β-glucosidase gene bgl1. All values were normalized to actin expression under same conditions. Data are the Mean ± SD of the results from three independent experiments. Significant differences were analyzed using t test. (*p < 0.05, **p < 0.01.)