Fig. 5

Cellulase production in the xyr1-A824V overexpressing strains QA1Xg and QA1Xc under glucose culture conditions. A Qualitative evaluation for the ability to secrete cellulase using the MM plate containing both 0.5% ball-milled Avicel and 1% glucose as carbon sources. B The activities of FPA and C the amount of extracellular protein at 3 days, 5 days, and 7 days of glucose culture conditions. D CBH activity, E EG activity and F BGL activity at 7 days under glucose culture conditions. G The transcript levels of the cellobiohydrolase genes cbh1 and cbh2 at 72 h. H The transcript levels of the endoglucanase genes eg1 and eg2 at 72 h. I The transcript level of the β-glucosidase gene bgl1 at 72 h. All values were normalized to actin expression under same conditions. Data are the Mean ± SD of the results from three independent experiments. Significant differences were analyzed using t test. (*p < 0.05, **p < 0.01)