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Fig. 8 | Biotechnology for Biofuels and Bioproducts

Fig. 8

From: An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production

Fig. 8

Production of glucose oxidase in the gox expressing strain QGOD. A The transcript levels of the UPR-related genes bip1 and pdi1 in QGOD and the parental strain QM53. B The transcript levels of the ERAD related genes hrd1 and der1 in QGOD and QM53. C SDS-PAGE analysis of the fermentation supernatants from QGOD and QM53 under lactose culture conditions. D The activities of glucose oxidase produced by QGOD at 3 days, 6 days and 9 days under lactose culture conditions. All values were normalized to actin expression under same conditions. Data are the Mean ± SD of the results from three independent experiments. Significant differences were analyzed using t test. (*p < 0.05, **p < 0.01.)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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