Fig. 2

Effect of site-directed mutation of DGATs on lipid and EPA synthesis. (a) The region of site-directed mutation in ScDGAT2A and ScDGAT2B. (b) Schematic diagram of site-directed mutation of ScDGAT2A and ScDGAT2B. (c) Sanger sequencing confirmation of site-directed mutation of ScDGAT2A and ScDGAT2B. Effects of site-directed mutation of ScDGAT2A and ScDGAT2B on lipid and DCW (d), fatty acid composition (e), C16:0 and C18:2 content (f), and UFAs and SFAs content (g) in Y. lipolytica mutant strain (CK). Three biological replicates were used and mean values ± SD (n = 3) are shown. Student’s t test was used for statistical analysis, and statistical significance is indicated as *P < 0.05, **P < 0.01, and ***p < 0.001