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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Identification of lipid synthesis genes in Schizochytrium sp. and their application in improving eicosapentaenoic acid synthesis in Yarrowia lipolytica

Fig. 3

Effects of DGATs on lipid and fatty acid synthesis in EPA-producing Y. lipolytica (named yl-EPA) strain. a Scheme of the expression of DGAT genes controlled by the TEFin promoter and CYC1t terminator at the intF3 integration site based on homologous recombination. intF3-Up, upstream homologous arm of integration site intF3; intF3-Down, downstream homologous arm of integration site intF3. Effects of DGATs on lipid and DCW (b), fatty acid composition (c), UFAs and SFAs content (d), and EPA content and titer (e) in yl-EPA strain. Three biological replicates were used and mean values ± SD (n = 3) are shown. Two-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ***p < 0.001 compared with the yl-EPA

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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