Fig. 4

Lipomics analysis of the effect of ScDGAT2C on EPA storage. a Schematic of the steps in the workflow of lipomics analysis. b Effects of ScDGAT2C on glycerolipids, glycerophospholipids, sphingolipids, diacylglycerol (DG), and Triglyceride (TG) in yl-EPA strain. c Volcano plot analysis of yl-EPA::ScDGAT2C and yl-EPA. Red represents up-regulated metabolites, blue represents down-regulated metabolites, and gray represents unchanged metabolites. d Effects of ScDGAT2C on EPA-related TG in yl-EPA strain. e Effects of ScDGAT2C on EPA-related DG, phosphatidylcholine (PC), Bis-methyl phosphatidic acid (BisMePA), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) in yl-EPA strain. (f) Clustering analysis of lipid metabolites with the top 50 variable importance (VIP) values under ScDGAT2C expression conditions. Each row of the color block gradient map on the left represents a metabolite, and the different colors represent the relative abundance of metabolites in the sample set. The right side is the VIP bar graph of metabolites, the length of the bar represents the contribution of the metabolite to the difference between two groups and the color of the bar indicates the significance of the difference. Three biological replicates were used and mean values ± SD (n = 3) are shown. Two-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ***p < 0.001 compared with the yl-EPA