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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Identification of lipid synthesis genes in Schizochytrium sp. and their application in improving eicosapentaenoic acid synthesis in Yarrowia lipolytica

Fig. 5

Effect of GPATs on lipid and EPA synthesis. a Schematic diagram of the introduction of GPATs to improve EPA storage. EPA eicosapentaenoic acid, G3P glycerol-3-phosphate, GPAT glycerol-sn-3-phosphate acyl-transferase, LPA lysophosphatidate, PA phosphatidate, DG diacylglycerol, DGAT diacylglycerol acyltransferase, TG triacylglycerol. b Phylogenetic analysis of amino acid sequences of ScGPAT1 and ScGPAT2. Effects of GPATs on lipid and DCW (c), fatty acid composition (d), PUFAs and SFAs content (e), and EPA content and titer (f) in yl-EPA::ScDGAT2C strain. g Volcano plot analysis of yl-EPA::ScDGAT2C::ScGPAT2 and yl-EPA::ScDGAT2C strains. Red represents up-regulated metabolites, blue represents down-regulated metabolites, and gray represents unchanged metabolites. h Effects of ScGPAT2 on EPA-related TG in yl-EPA::ScDGAT2C strain. Three biological replicates were used and mean values ± SD (n = 3) are shown. Two-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ***p < 0.001 compared with the yl-EPA::ScDGAT2C

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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