Fig. 1

Feasibility validation of NHEJ-mediated gene regulatory library for gene activation. A Schematic diagram of constructing genome-scale gene regulatory library by NHEJ-mediated random integration. B Schematic diagram of the effect of different insertion positions of the LEU2-UT8 fragment on gene expression. When the LEU2-UT8 DNA fragment is inserted into the genomic promoter (Pro) region with the same 5’-3’ direction, it may activate gene expression. When it is inserted in the opposite direction, it may disrupt the function of the endogenous promoter, leading to the repression of downstream genes. Insertion within a gene will disrupt gene expression. C Schematic diagram of inserting the UT8 promoter into different positions of the DGA1 promoter to control hrGFP expression. D Effect of different positions of UT8 promoter inserted into DGA1 promoter on hrGFP expression