Fig. 3

Identification of mutants with activated xylose metabolism in Y. lipolytica. A Insertion position (red triangle) of the LEU2-UT8 fragment in the mutants. B, C Spot assay B and growth curves C of the control strain Y. lipolytica Po1f and the mutant strains with xylose as the sole carbon source. D Relative transcription levels of YALI1_C28456g. E Schematic diagram of genome modification of different mutants. F Growth curves of YALI1_C28456g overexpression and knockout mutant strains with xylose as the sole carbon source. G Redefinition of the YALI1_C28456g ORF. XylK2: library mutant strain with insertion of the LEU2-UT8 fragment at the 33-bp position of YALI1_C28456g. Xylall: strain with UT8 promoter-driven overexpression of YALI1_C28456g. Xyl303: strain with overexpression of YALI1_C28456g carrying a deletion of the 5′ 303 bp. XylΔall: YALI1_C28456g knockout strain. XylΔ33: strain with deletion of the 5′ 33 bp of YALI1_C28456g