Fig. 1

Phylogenetic analysis and sequence alignment of AA14 LPMOs. A Phylogenetic tree of putative AA14 LPMOs and characterized AA9 LPMOs. The sequences encoding signal peptide, CBM1 and the dCTR based on the prediction using MobiDB-lite 5.0 (https://mobidb.bio.unipd.it/) in selected genes were deleted, and only the catalytic domain was selected for phylogenetic analysis. Blue dot indicates proteins from Basidiomycota, while red dot indicates proteins from Ascomycota B Sequence alignment of TrAA14A and 14B with PcAA14A and 14B. The amino acid residues forming the His brace are indicated as the solid red arrow