Fig. 2
HPAEC-PAD analysis of reaction products generated by TrAA14A from RAC-85 and xyloglucan. A The reaction products generated from RAC-85. B The reaction products generated from xyloglucan. The oxidative activities of TrAA14A towards cellulosic substrates or xyloglucan were determined in the reaction mixture (2.0 mL) containing various substrates (5 mg), 1 μM TrAA14A and 1 mM AscA in sodium acetate buffer (pH 5.0, 50 mM) in an incubator at 45 ℃ and 200 rpm for 24 h. The control reaction containing various substrates (5 mg) with AscA (1 mM), or AscA (1 mM) and Cu2+ (1 μM), or heat-inactivated TrAA14A (designated TrAA14A(boiled), boiled at 99 °C for 15 min) (1 μM) and AscA (1 mM) was also performed in parallel under the same condition