Fig. 3

MALDI-TOF MS analysis of the products from RAC-85 (A) and xyloglucan (B) by TrAA14A. nonoxidized and oxidized products are shown in blue and red letters, respectively. Hex, hexose; Pen, pentose; OX, C4 oxidized product; OX*, C1-oxidized product in the aldonic acid form or C4-oxidized product in the gemdiol form. The oxidative reaction of TrAA14A towards RAC-85 and xyloglucan was performed in the reaction mixture (2.0 mL) containing various substrates (5 mg), 1 μM TrAA14A and 1 mM AscA in sodium acetate buffer (pH 5.0, 50 mM) in an incubator at 45 ℃ and 200 rpm for 24 h