Fig. 4

The synergy between TrAA14A and GHs on cellulosic substrates. A The released reducing sugar from the synergy between TrAA14A and GHs on mercerized fiber. B The released reducing sugar from the synergy between TrAA14A and GHs on RAC-85. C The degree of synergy (DS) for TrAA14A with GHs on mercerized fiber. D The degree of synergy (DS) for TrAA14A with GHs on RAC-85. The synergism of TrAA14A with GHs was performed in a reaction mixture (1 mL) by mixed incubation of TrAA14A (1 μM) with EGI (10 μg), CBHI (20 μg), CBHII (20 μg), or Celluclast®1.5L (0.04 U), and 4 mg/mL RAC-85 or mercerized fiber, with 1 mM AscA in sodium acetate buffer (pH 5.0, 50 mM) at 45 ℃ and 1000 rpm for 1 h in a thermomixer. The control reaction in a reaction mixture (1 mL) containing individual TrAA14A (1 μM), EGI (10 μg), CBHI (20 μg), CBHII (20 μg), or Celluclast®1.5L (0.04 U), and 4 mg/mL RAC-85 or mercerized fiber, with 1 mM AscA was performed under same condition