Fig. 5

HPAEC-PAD analysis of reaction products generated by TrAA14A from different hemi/cellulosic substrates. A PFI-fibrillated eucalyptus pulp. B β-Cellulose. C Xylan-10%. D Xylan-5%. The reaction was performed in the reaction mixture (500 μL) containing 5 mg substrate, TrAA14A (1 μM) and AscA (1 mM) in sodium acetate buffer (pH 5.0, 50 mM) in a thermomixer at 45 ℃ and 1000 rpm for 1 h. The control reaction containing various substrates (5 mg) with AscA (1 mM), or AscA (1 mM) and Cu²⁺ (1 μM), or heat-inactivated TrAA14A [designated TrAA14A(boiled), boiled at 99 °C for 15 min] (1 μM) and AscA (1 mM) was also performed in parallel under the same condition. After reaction, the reaction solution was immediately filtered by passing the reaction mixture through a membrane with a pore size of 0.22 μm and analyzed by HPAEC-PAD