Fig. 6
MALDI-TOF analysis of the products generated by TrAA14A from PFI-fibrillated eucalyptus pulp. Blue: Cello-oligos; Dark blue: Oxidized cello-oligos. Light green: Xylo-oligos; Green: Oxidized xylo-oligos. Red: Xylo-oligos with methyl glucuronic acid; Dark red: Oxidized xylo-oligos with methyl glucuronic acid. Purple: XG-oligos; Dark purple: Oxidized XG-oligos. All labelled peaks indicate monocharged sodiated molecules. OX: C4 oxidized products; OX*−2: C1 and C4 double oxidation products. OX*: C1-oxidized product in the aldonic acid form or C4-oxidized product in the gemdiol form. #: Sodium salts of aldonic or alduronic acids. The reaction was performed in the reaction mixture (500 μL) containing 5 mg substrate, TrAA14A (1 μM) and AscA (1 mM) in sodium acetate buffer (pH 5.0, 50 mM) in a thermomixer at 45 ℃ and 1000 rpm for 1 h