Fig. 7

Comparison of structure and electrostatic potential of TrAA14A predicted by Alphafold 2 with PcAA14B (PDB ID: 5NO7) and the previously characterized xylan-active NcLPMO9F (PDB ID: 4QI8). A The three-dimensional structure of TrAA14A PcAA14B and NcLPMO9F in two views related by a 90° rotation to visualize of the loop, helix and sheet decorated by white, pink and slate carton, respectively. B Comparison of TrAA14A (gray and green), PcAA14B (gray and violet) and NcLPMO9F (gray and slate), and displayed in two views related by a 90° rotation. C Active site residues overlay of TrAA14A (green), PcAA14B (violet), and NcLPMO9F (slate). D, E The electrostatic potential calculated for TrAA14A, PcAA14B and NcLPMO9F at pH 5.0, and pH 6.0, respectively. The electrostatic potential is shown as color gradient from red to blue (− 5 to + 5 kT/e), and the catalytic center Cu is shown as brown sphere. The gray circle indicates the L2, and the green circle indicates the L3