Fig. 10

Conversions of galactomannan amination catalyzed by FgrGaOx and SpATA mutants measured using the Q-NPEA assay. The dashed line indicates the conversion achieved by the wild-type SpATA in 30 h, and percentages indicate how 30-h conversions achieved by SpATA variants compare to the wild-type SpATA. Reactions (200 µL) consisted of 50 mM HEPES buffer (pH 7.5), 29.8 µg/mL FgrGaOx, 12.8 µg/mL catalase, 1.8 µg/mL HRP, 0.15 mg/mL ATA, 10 mM NPEA, 20 µM PLP and 0.29% galactomannan containing 5 mM galactose. Reactions were conducted at 37 °C and 600 rpm on a 96-well plate (n = 4, error bars indicate standard errors)