Fig. 4

Conversions of FgrGaOx-oxidized carbohydrates through amination by CvATA (blue) and SpATA (orange) measured using the Q-NPEA assay. The oxidation and transamination reactions were performed simultaneously in one pot. The inserted line graph shows the amination of oxidized galactose with a linear time scale to underscore the higher initial activity of CvATA. Reactions (200 µL) comprised 50 mM HEPES buffer (pH 7.5), 29.8 µg/mL FgrGaOx, 12.8 µg/mL catalase, 1.8 µg/mL HRP, 150 µg/mL ATA, 20 µM PLP, 10 mM NPEA and carbohydrates containing 5 mM galactose and were carried out at 37 °C and 600 rpm. (n = 4, error bars indicate standard errors)