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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Biocatalytic cascade to polysaccharide amination

Fig. 5

ESI-Q-TOF MS spectra (positive ionization mode) of aminated galactose produced via CvATA and SpATA treatments with simultaneous FgrGaOx oxidation. The relative abundance of aminated galactose was quantified using HEPES as an internal reference. Reactions (1 mL) comprised 50 mM HEPES (pH 7.5), 29.8 µg/mL FgrGaOx, 1.8 µg/mL HRP, 12.8 µg/mL catalase, 0.15 mg/mL ATA, 20 µM PLP, 5 mM (S)-1-phenylethylamine and 5 mM galactose were carried out at 37 °C and 600 rpm for 5 h

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