Fig. 7

Conversions of galactomannan amination catalyzed by FgrGaOx with reduced doses and SpATA measured using the Q-NPEA assay. Precipitate formation were measured separately from the transparent adhesive film and the liquid suspension. Reactions (200 µL) comprised 50 mM HEPES buffer (pH 7.5), 20 µM PLP, 10 mM NPEA, 0.15 mg/mL SpATA, 0.29% galactomannan equivalent to 5 mM galactose and FgrGaOx at various doses. The 1.0 × FgrGaOx dose corresponds to 29.8 µg/mL FgrGaOx, 12.8 µg/mL catalase, and 1.8 µg/mL HRP. All reactions were carried out at 37 °C and 600 rpm on 96-well plates (n = 4, error bars indicate standard errors)